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Canine Brucellosis in the Netherlands : first local cases

Published on 13/05/2019

[relayed from Promed] The Dutch Food and Consumer Product Safety Authority (NVWA) has found for the 1st time, the bacterium _Brucella canis_ in dogs born in the Netherlands.

CDC Advisory: Third Case of Rifampin/Penicillin-Resistant Strain of RB51 Brucella from Consuming Raw Milk

Published on 27/01/2019

[relayed from CDC Health Alert Network] The New York State Department of Health and Pennsylvania Department of Health are investigating Brucella RB51 exposures that may be connected to consuming raw (unpasteurized) milk from Miller’s Biodiversity Farm in Quarryville, Pennsylvania. Symptoms of brucellosis can include fever, sweats, malaise, anorexia, headache, fatigue, muscle and joint pain, and potentially more serious complications (e.g., endocarditis, hepatomegaly, splenomegaly, and neurologic symptoms). In pregnant patients, Brucella infections can be associated with miscarriage. Symptom onset can occur anywhere from five days to six months following exposure. As of January 22, 2019, exposures have been identified in 19 states: Alabama, California, Connecticut, Florida, Georgia, Iowa, Maryland, Massachusetts, Michigan, Minnesota, Mississippi, New Jersey, New York, North Carolina, Ohio, Pennsylvania, Rhode Island, South Carolina, and Virginia.

Merry Christmas and happy New Year !

Published on 21/12/2018

Wishing you all the timeless treasures of Christmas… The warmth of home, the love of family and the company of good friends. The EURL Team

European Union summary report on trends and sources of zoonoses, zoonotic agents and food‐borne outbreaks in 2017

Published on 12/12/2018

[relayed from EFSA & ECDC] This report of the European Food Safety Authority and the European Centre for Disease Prevention and Control presents the results of zoonoses monitoring activities carried out in 2017 in 37 European countries (28 Member States (MS) and nine non‐MS).

The EURL team is attending the International Brucellosis Society Meeting in Chicago

Published on 02/12/2018

The EURL team is attending the International Brucellosis Society Meeting, taking place in Chicago this weekend (December 2 & 3). Guillaume Girault and Luca Freddi presented two oral communications on the development of our High Resolution Melting PCR tool and on our recent investigations in domestic frogs respectively. Both slide presentations will be added to the EURL website for the NRLs network. A summary of the main findings presented during the conference will be reported in our next EURL Newsletter. 

First International Symposium on Brucellosis in Beijing

Published on 11/11/2018

On October 31 and November 1st, IVDC (Institute for Vet Drug Control) organised the First International Symposium on Brucellosis in Beijing, regrouping more than 250 scientists, stakeholders together with international experts from France (P Boireau, C Ponsart), Ethiopia (A Gelaw), Germany (H Neubauer), Israël (M Banai) and USA (Oliver He). During the opening ceremony, IVDC has been nominated as National Reference Lab for Brucellosis. The meeting provided very interesting updated information on the Chinese epidemiological  situation, research projects and control strategies.

Practical training session at EURL for Brucellosis: Bacteriology on the menu !

Published on 10/10/2018

On the 4th and 5th of October, a group of 5 participants from the European network of reference laboratories for Brucellosis were welcomed at ANSES, Animal Health Laboratory for a 2-day training session in bacteriology. Representatives from Croatia, Norway, Portugal, Slovenia and Spain were trained to working conditions in the Biosafety level 3 laboratory and to phenotypical typing techniques. Each participant had the opportunity to practice preparation of samples (lymph nodes) and performed main bacteriological tests to identify a panel of 5 different Brucella strains. The EURL team prepared different culture isolates in order to demonstrate growth characteristics, culture on dyes and phage lyses profiles. The administrative and logistical aspects were ensured by Marie-France Devaux. The technical demonstrations and practical work were done by Ludivine Perrot and Benoît Taunay, assisted by Luca Freddi and Claire Ponsart. As with each training session of the EURL for Brucellosis, a guided tour of the Fragonard Museum was organized at the end of the first day to raise awareness of the veterinary heritage of the Alfort Vet Campus.

11th Workshop at ANSES: a broad programme on atypical Brucella, Animal Health Law and serological diagnosis in Swine

Published on 22/09/2018

The 11th Workshop was held in Maisons-Alfort on 20 et 21st of September. A total of 28 countries including France were represented. Two invited speakers contributed to 2 major topics during the workshop: 1) Alexandre Fediaevsky (DG SANTE, Brussels) highlighted main steps on Animal Health Law implementation and consequences on Brucellosis surveillance and control in Europe ; 2) Sascha Al Dahouk (BfR, Berlin) presented updates on atypical Brucella and especially recent data relative to Amphibian isolates. Six National Reference Laboratories (NRLs) including France presented oral communications on brucellosis serological diagnosis in swine, Brucellosis in frogs and the Bulgarian representative described the Brucellosis situation and associated reference activities from his country. Many thanks to all contributors and participants! Many perspectives were discussed during both days to prepare the 2019-2020 Workprogramme, including reagents and standarisation of Brucella ovis diagnostic, new kits available for porcine brucellosis diagnostic, implementation of genomic tools and changes related to the future Anmal Health Law. Reports and presentations will be soon available on the website. Looking forward to working further together on these topics of comon interest ! The EURL team

Recent paper: Core Genome Multi Locus Sequence Typing and Single Nucleotide Polymorphism Analyses in the Epidemiology of Brucella melitensis Infections

Published on 23/06/2018

Keywords : WGS analyses of B. melitensis infection

Authors Anna Janowicz, Fabrizio De Massisa, Massimo Ancoraa, Cesare Cammàa, Claudio Patavinoa, Antonio Battistib, Karola Priorc, Dag Harmsenc, Holger Scholzd, Katiuscia Zillia, Lorena Sacchinia, Elisabetta Di Giannatalea and Giuliano Garofolo Abstract [relayed from jcm.asm.org] The use of whole genome sequencing (WGS) using next generation sequencing (NGS) technology has become a widely accepted method for microbiology laboratories in the application of molecular typing for outbreak tracing and genomic epidemiology. Several studies demonstrated the usefulness of WGS data analysis through single nucleotide polymorphism (SNP) calling from a reference sequence analysis for Brucella melitensis, whereas gene-by-gene comparison through core-genome multilocus sequence typing (cgMLST) has not been explored so far. The current study developed an allele-based cgMLST method and compared its performance to the genome-wide SNP approach and the traditional MLVA on a defined sample collection. The dataset comprised of 37 epidemiologically linked animal cases of brucellosis as well as 71 isolates with unknown epidemiological status composed of human and animal samples collected in Italy.

New! LEILA, a software to simplify organisation of Inter-Laboratory Proficiency tests !

Published on 18/06/2018

Dear colleagues,   Anses has developed a software/web interface (https://leila.anses.fr/) to simplify organisation of Inter-Laboratory Proficiency tests ! This software will facilitate the access to all the information concerning the ILPT (calendar, online registration, documents, reports). Each laboratory has now to create a Laboratory workspace into the software. You can find a tutorial to create your workspace (https://vimeo.com/ansesleila). After this creation, you will be ready for the future ILPT. Hope to see you soon on LEILA!   In case of any further question, please contact us (eurl.brucellosis@anses.fr)