Published on 22/09/2018
The 11th Workshop was held in Maisons-Alfort on 20 et 21st of September. A total of 28 countries including France were represented. Two invited speakers contributed to 2 major topics during the workshop: 1) Alexandre Fediaevsky (DG SANTE, Brussels) highlighted main steps on Animal Health Law implementation and consequences on Brucellosis surveillance and control in Europe ; 2) Sascha Al Dahouk (BfR, Berlin) presented updates on atypical Brucella and especially recent data relative to Amphibian isolates.
Six National Reference Laboratories (NRLs) including France presented oral communications on brucellosis serological diagnosis in swine, Brucellosis in frogs and the Bulgarian representative described the Brucellosis situation and associated reference activities from his country.
Many thanks to all contributors and participants!
Many perspectives were discussed during both days to prepare the 2019-2020 Workprogramme, including reagents and standarisation of Brucella ovis diagnostic, new kits available for porcine brucellosis diagnostic, implementation of genomic tools and changes related to the future Anmal Health Law. Reports and presentations will be soon available on the website.
Looking forward to working further together on these topics of comon interest !
The EURL team
Published on 23/06/2018
Keywords : WGS analyses of B. melitensis infection
Anna Janowicz, Fabrizio De Massisa, Massimo Ancoraa, Cesare Cammàa, Claudio Patavinoa, Antonio Battistib, Karola Priorc, Dag Harmsenc, Holger Scholzd, Katiuscia Zillia, Lorena Sacchinia, Elisabetta Di Giannatalea and Giuliano Garofolo
Abstract [relayed from jcm.asm.org]
The use of whole genome sequencing (WGS) using next generation sequencing (NGS) technology has become a widely accepted method for microbiology laboratories in the application of molecular typing for outbreak tracing and genomic epidemiology. Several studies demonstrated the usefulness of WGS data analysis through single nucleotide polymorphism (SNP) calling from a reference sequence analysis for Brucella melitensis, whereas gene-by-gene comparison through core-genome multilocus sequence typing (cgMLST) has not been explored so far. The current study developed an allele-based cgMLST method and compared its performance to the genome-wide SNP approach and the traditional MLVA on a defined sample collection. The dataset comprised of 37 epidemiologically linked animal cases of brucellosis as well as 71 isolates with unknown epidemiological status composed of human and animal samples collected in Italy.
Published on 18/06/2018
Anses has developed a software/web interface (https://leila.anses.fr/) to simplify organisation of Inter-Laboratory Proficiency tests !
This software will facilitate the access to all the information concerning the ILPT (calendar, online registration, documents, reports).
Each laboratory has now to create a Laboratory workspace into the software. You can find a tutorial to create your workspace (https://vimeo.com/ansesleila).
After this creation, you will be ready for the future ILPT.
Hope to see you soon on LEILA!
In case of any further question, please contact us (email@example.com)
Published on 15/06/2018
The EURL team organised the first 2018 training session on the 14th and 15th June at ANSES. This 2-day training session focused on control of ELISAs and Rose Bengale (RB) antigens. Eight participants from Croatia, Ireland, Lithuania, Malta, Portugal, Romania, Slovenia and Spain performed a successful control of a serum ELISA kit and two RB antigens. It was a good opportunity to exchange on critical points of reagents controls and share experience on protocols and reagents.
Published on 08/06/2018
Keywords : Brucella - MALDI-TOF mass spectrometry
Sali M, De Maio F, Tarantino M, Garofolo G, Tittarelli M, Sacchini L, et al.
[Relayed from journals.plos.one] Brucellosis is essentially a disease of domesticated livestock; however, humans can also be infected via the consumption of contaminated meat or dairy products, underlying the need for rapid and accurate identification methods. Procedures for microbiological identification and typing of Brucella spp. are expensive, time-consuming, and must be conducted in biohazard containment facilities to minimize operator risk. The development of a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS)-based assay has reduced the processing time while maintaining performance standards. In this study, to improve the identification accuracy and suitability of the MALDI-TOF-based assay for routine diagnosis, we developed a new protein extraction protocol and generated a custom reference database containing Brucella strains representative of the most widespread species. The reference library was then challenged with blind-coded field samples isolated from infected animals. The results indicated that the database could be used to correctly identify 99.5% and 97% of Brucella strains at the genus and species level, respectively, indicating that the performance of the assay was not affected by the different culture conditions used for microbial isolation. Moreover, the inactivated samples were stored and shipped to reference laboratories with no ill effect on protein stability, thus confirming the reliability of our method for routine diagnosis. Finally, we evaluated the epidemiological value of the protocol by comparing the clustering analysis results of Brucella melitensis strains obtained via multiple locus variable-number tandem repeat analysis or MALDI-TOF MS. The results showed that the MALDI-TOF assay could not decipher the true phylogenetic tree, suggesting that the protein profile did not correspond with the genetic evolution of Brucella.
Published on 30/05/2018
Keywords : Brucella melitensis - wildlife - Capra ibex
Wildlife reservoirs of infectious diseases raise major management issues. In Europe, brucellosis has been eradicated in domestic ruminants from most countries and wild ruminants have not been considered important reservoirs so far. However, a high prevalence of Brucella melitensis infection has been recently identified in a French population of Alpine ibex (Capra ibex), after the emergence of brucellosis was confirmed in a dairy cattle farm and two human cases. This situation raised the need to identify the factors driving the persistence of Brucella infection at high prevalence levels in this ibex population. In the present paper, we studied the shedding pattern of B. melitensis in ibex from Bargy Massif, French Alps. Bacteriological examinations (1–15 tissues/samples per individual) were performed on 88 seropositive, supposedly infected and euthanized individuals. Among them, 51 (58%) showed at least one positive culture, including 45 ibex with at least one Brucella isolation from a urogenital sample or a lymph node in the pelvic area (active infection in organs in the pelvic area). Among these 45 ibex, 26 (30% of the total number of necropsied animals) showed at least one positive culture for a urogenital organ and were considered as being at risk of shedding the bacteria at the time of capture. We observed significant heterogeneity between sex-and-age classes: seropositive females were most at risk to excrete Brucella before the age of 5 years, possibly corresponding to abortion during the first pregnancy following infection such as reported in the domestic ruminants. The high shedding potential observed in young females may have contributed to the self-sustained maintenance of infection in this population, whereas males are supposed to play a role of transmission between spatial units through venereal transmission during mating. This heterogeneity in the shedding potential of seropositive individuals should be considered in the future to better evaluate management scenarios in this system as well as in others.
Published on 27/05/2018
The 86th OIE General Session was the occasion to give OIE Member Countries, an overview of the global animal health situation, as well as to analyse trends in the evolution of priority terrestrial and aquatic animal diseases. In addition, new disease status were officially recognised for 10 countries (source OIE).
Follow the discussions of the 86th OIE General Session on social media :#86SG
Published on 25/05/2018
Published on 25/05/2018
Keywords : recent publication
A Brucella suis biovar 1 infection was diagnosed in a dog without typical exposure risks, but the dog had been fed a raw meat-based diet (hare carcasses imported from Argentina).
Track and trace investigations revealed that the most likely source of infection was the dog's raw meat diet.